Tobey Maguire

Spider-Man / Peter Parker

Kirsten Dunst

Mary Jane Watson

James Franco

New Goblin / Harry Osborn

Thomas Haden Church

Sandman / Flint Marko

Topher Grace

Venom / Eddie Brock

Bryce Dallas Howard

Gwen Stacy

Rosemary Harris

May Parker

J.K. Simmons

J. Jonah Jameson

James Cromwell

Captain Stacy

Dylan Baker

Dr. Curt Connors

Theresa Russell

Emma Marko

Bill Nunn

Joseph 'Robbie' Robertson

Bruce Campbell

Maître D'

Elizabeth Banks

Miss Brant

Ted Raimi

Hoffman

Perla Haney-Jardine

Penny Marko

Willem Dafoe

Green Goblin / Norman Osborn

Cliff Robertson

Ben Parker

Elya Baskin

Mr. Ditkovitch

Mageina Tovah

Ursula