Tobey Maguire

Spider-Man / Peter Parker

Kirsten Dunst

Mary Jane Watson

James Franco

Harry Osborn

Alfred Molina

Doc Ock / Otto Octavius

Rosemary Harris

May Parker

J.K. Simmons

J. Jonah Jameson

Donna Murphy

Rosalie Octavius

Daniel Gillies

John Jameson

Dylan Baker

Dr. Curt Connors

Bill Nunn

Joseph 'Robbie' Robertson

Vanessa Ferlito

Louise

Aasif Mandvi

Mr. Aziz

Willem Dafoe

Green Goblin / Norman Osborn

Cliff Robertson

Ben Parker

Ted Raimi

Hoffman

Elizabeth Banks

Miss Brant

Bruce Campbell

Snooty Usher

Gregg Edelman

Dr. Davis

Elya Baskin

Mr. Ditkovitch

Mageina Tovah

Ursula